Primer

Part:BBa_K5269012

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)


Golden Gate Assembly Site In-Fusion reverse primer 1

This reverse primer is used for Golden Gate Assembly Site (BBa_K5269020), together with Golden Gate Assembly Site In-Fusion forward primer 1 (BBa_K5269011) for PCR and In-Fusion of Golden Gate Assembly Site (BBa_K5269020) to pBluescript II SK(-). Used for PCR and In-Fusion of Golden Gate Assembly Site (BBa_K5269020) to pBluescript II SK(-).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
    Illegal SpeI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 16
    Illegal NotI site found at 2
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
    Illegal SpeI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
    Illegal SpeI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None